Chlamydomonas hap2
WebChlamydomonas HAP2 is a member of a broadly conserved protein family whose founding member was identified in Arabidopsis in a screen for male sterile mutants (Johnson et al., 2004). [HAP2 more recently has also been termed GCS1 (Mori et al., 2006).] Previously, using gene disruption methods, we showed that in both Chlamydomonas and the rodent WebJun 15, 2024 · The kinetics of degradation of HAP2-GCS1 and FUS1 in Chlamydomonas zygotes (mentioned above) are consistent with a process activated by fresh transcription after fusion, at least in part . Remarkably, although land plants have dramatically elaborated lifecycles compared to their green algal ancestors, this ancestral function of …
Chlamydomonas hap2
Did you know?
WebDec 31, 2016 · Structural modeling of the HAP2/GCS1 protein family predicts that they are homologous to EFF-1 and class II fusion proteins from enveloped viruses (e.g. dengue and Zika viruses). WebAug 13, 2024 · HAPLESS2 (HAP2) is a broadly conserved, gamete-expressed transmembrane protein that was shown recently to be structurally homologous to viral …
WebJul 9, 2024 · The cellular fusion protein HAP2, which is structurally homologous to viral class II fusion proteins, drives gamete fusion across several eukaryotic kingdoms. Gamete … WebFeb 28, 2024 · X-ray crystallography reveals that two closely related proteins, the HAP2 protein from the single-celled alga Chlamydomonas reinhardtii and the fusion protein from dengue virus are essential for fusion with a cell membrane and thus both sexual reproduction and viral invasion of a host cell. The proteins probably emerged very early …
WebFeb 23, 2024 · Studies in Chlamydomonas and Plasmodium (the pathogen causing malaria in humans) revealed that hap2 mutant gametes in both organisms were fully capable of … WebFeb 5, 2015 · In Chlamydomonas, HAP2 is constit utively prese nt on the cel l. surface at a specialized, apically localized membrane patch, the. mating structure (Fig. 1), the surfa ce area of which repr esents ...
WebDec 20, 2024 · Furthermore, comparisons between the HAP2 structures of Chlamydomonas, Arabidopsis, and Trypanosoma cruzi showed substantial, taxa …
WebJan 10, 2024 · HAP2/GCS1 proteins share a similar structure with two distinct families of exoplasmic fusogens: the somatic Fusion Family (FF) proteins discovered in nematodes, … hach filter moduleWeb1 Introduction. Chlamydomonas is an excellent model system to study the regulation of cilia and flagella. All major structural components of cilia are conserved in this unicellular … brad tyson obituaryWebOct 1, 2024 · The first atomic model of HAP2 [27], a Chlamydomonas HAP2 homotrimer, revealed an unambiguous structural homology to class II fusion proteins, with the β sheet-rich domains I, II, and III arranged in the folded-back (hairpin) conformation typical of the viral proteins in the post-fusion trimer [17]. In their prefusion form on virus particles ... brad turner twitterWebMay 3, 2024 · HAP2 is a class II gamete fusogen in many eukaryotic kingdoms. A crystal structure of Chlamydomonas HAP2 shows a trimeric fusion state. Domains D1, D2.1 … hach filtertrak 660 sc manualWebMay 6, 2024 · The screening experiment with pJPZ11_HAP2 did not resulted in any positive candidate, the outcome was confirmed by 50 mL cultures of the top candidate (Supp. Figure S.2). The maximum RFU obtained during the screening in the 96 well plates for pJPZ11_HAP2 (3181) was similar to the RFU observed for the wild-type strain (3068). brad tyson anderson scWebThe structure of Chlamydomonas HAP2 suggests that the cd loop is bipartite, with the two parts split by a small alpha helix (Fe´dry et al., 2024). Given this specific structure/function biological activity observed during the study of HAP2 in Tetrahymena, Arabidopsis, and Chlamydomonas, and the general conservation of the protein hach filterWebHAP2 localizes at the fusion site of Chlamydomonas minus gametes, yet Chlamydomonas minus and Plasmodium hap2 male gametes retain the ability, using … brad tyson neuropsychology