WebMar 26, 2024 · Here we focus on antibody class switch recombination (CSR), and review the initiation of base damages, the conversion of damaged base to DNA double-strand break, and the ligation of broken ends. With an emphasis on the unique features in CSR, we discuss recent advances in the understanding of DNA repair/replication coordination, and … WebJul 30, 2012 · Immunoglobulin class-switch recombination deficiencies (Ig-CSR-Ds) are rare primary immunodeficiencies characterized by defective switched isotype (IgG/IgA/IgE) production. Depending on the molecular defect in question, the Ig-CSR-D may be combined with an impairment in somatic hypermutation (SHM).
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WebSchematic representation of PI3Kδ activation in B- and T-cells during class-switch recombination. In B cells, PI3Kδ is activated mainly after the BCR (B-cell receptor) cross … Webclass switch recombination CTCF CCCTC-Binding Factor DDX1 DEAD-box RNA helicase 1 DNA-PK DNA protein kinase DNA-PKcs DNA-dependent protein kinase catalytic subunit DSB double-strand breaks Ebf1 early B-cell factor 1 eEF1A eukaryotic elongation factor 1 α Erag enhancer region of RAG1/2 Exo1 exonuclease 1 FOXO Forkhead box G4 G-quadruplexes … diamond polisher
Immunoglobulin class switching: in vitro induction and analysis
WebDec 14, 2012 · Class switch recombination is an irreversible process that can occur repeatedly until the immunoglobulin heavy chain repertoire is exhausted in a given cell. The regulation and execution of class switch recombination is dependent on the combined activity of protein complexes, chromosomal restructuring and DNA transcription [8]. WebSomatic hypermutation and class-switch-recombination are initiated by the deamination of deoxycytosine in DNA by activation-induced-deaminase, AID. Recently, there has been much research into how AID targets double-stranded DNA in sub-regions of Ig genes, the involvement of co-factors and posttranslational modifications in this process, the co … WebAug 11, 2016 · They then found that Polθ promotes class-switch recombination in naive B lymphocytes when NHEJ is suppressed . For example, insertions >1 bp at repair junctions were shown to be dependent on Polθ and this effect was more evident when NHEJ was suppressed by inhibition of DNA-PKcs. which is a signature of Polθ-mediated end-joining. cisco access point flashing green