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Pcr topo blunt

Splet质粒载体网zlzt.com专业提供pCR-BluntII-TOPO-CDK8(human),产品编号为zl-007888,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完美质粒众筹,质 … SpletZero Blunt® TOPO® PCR Cloning Kit for Sequencing . Five-minute cloning of blunt-end PCR products for sequencing . Catalog Numbers K2835-20, K2875-J10, K2875-20, K2875-40, K2880-20, K2880-40, K2895-20. Document Part Number 250277 . Publication Number MAN0000110 . Revision B.0 . 25% more. Now with TOPO reactions! USER GUIDE For …

pCR-BluntII-TOPO-LINC00597(human)(有突变)-质粒载体-ATCC …

Spletabove to produce your blunt-end PCR product. 2. Use agarose gel electrophoresis to check the integrity and determine the yield of your PCR product. Perform the TOPO® ® Cloning Reaction 1. Set up the following TOPO Cloning reaction. For optimal results, use a 0.5:1 to 2:1 molar ratio of PCR product:TOPO® vector. Reagent Chemical Transformation SpletpCR-BluntII-TOPO (Invitrogen, 450245) Zero Blunt TOPO PCR Cloning Kit. Map. iphone default ringtone bpm https://airtech-ae.com

TOPO Cloning of blunt-end PCR products_实验方法 - Everlab

SpletTopoisomerase-based cloning (TOPO cloning) is a molecular biology technique in which DNA fragments are cloned into specific vectors without the requirement for DNA ligases. Taq polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3'-end of the PCR products. SpletpCR-Blunt II-TOPO (linearized) Linearized vector for TOPO® cloning of blunt PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free … SpletZero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit • Eco R I sites flanking the PCR product insertion … iphone defined

Zero Blunt™ PCR Cloning Kit - Thermo Fisher Scientific

Category:pCR -Blunt II- TOPO - Thermo Fisher Scientific

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Pcr topo blunt

A universal cloning vector using vaccinia topoisomerase I

SpletWhen it comes to cloning, TOPO™ cloning technology has been a reliable partner for thousands of scientists for over ten years. Fast, simple-to-use, and efficient, TOPO™ … SpletpCR4-TOPO Parental sequencing vector for TOPO® TA cloning of PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free Download …

Pcr topo blunt

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SpletBlunt-end ligations require an excess of insert compared to your vector. 2. You also need to use a high concentration of ligase. 3. Blunt-end ligase reactions are inhibited by ATP... SpletThe Zero Blunt ® TOPO ® PCR Cloning Kit for Sequencing provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO ® Cloning") for the direct insertion of blunt-end …

Splet丁香通为您找到144条m13引物pcr设置厂家的工商注册年份、员工人数、年营业额、信用记录、主营产品、相关m13引物pcr设置产品的供求信息、交易记录等企业详情。丁香通为买家提供用户服务，诚信保障等服务，批发采购m13引物pcr设置，上丁香通。 SpletComments for pCR®-Blunt II-TOPO® 3519 nucleotides lac promoter/operator region: bases 95-216 M13 Reverse priming site: bases 205-221 LacZ-alpha ORF: bases 217-576 SP6 …

SpletTOPO PCR Cloning Kit (Cat. no. 450245) is shipped with only the Zero Blunt ™TOPO PCR Cloning reagents (Box 1). Box Store at 1 −30°C to −10°C in a non-frost-free freezer 2 … SpletZero Blunt™ PCR 克隆试剂盒有多种配置可供选择：含 One Shot™ TOPO10 化学感受态 大肠杆菌 （K2700-20 和 K2700-40）以及不含感受态细胞（K2750-20 和 K2750-40），有 20 次和 40 次反应试剂盒规格。 For Research Use Only. Not for use in diagnostic procedures. 规格 细菌或酵母菌株 TOP10 克隆方法 Blunt PCR 产品规格 试剂盒 产品线 Zero Blunt™ …

Splet质粒载体网zlzt.com专业提供pCR-BluntII-TOPO-GPR107(human),产品编号为zl-002714,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完美质粒众筹,质粒合成,质粒构建,质粒提取,质粒保藏!

SpletLabpO TOPO-TA/Blunt通用克隆试剂盒 (不含多克隆酶切位点MCS) 产品货号：PT0102 储存温度：－20℃储存，至少12个月。 冰袋运输，1-2 天置于室外常温不影响质量。 产品介绍： 本制品和传统的T4连接酶原理不同，它利用了Topoisomerase可以在瞬间（几sec-几min）、高效（接近100%）连接DNA片段的原理采用本公司独创的工艺制成。 （1）可以在瞬 … iphone default mail app office 365 mfaSpletTOPO vectors provide a fast and convenient method to reliably ligate PCR products (where 5′ ends are not phosphorylated) in as little as 5 minutes at room temperature. TOPO cloning utilizes the Vacinnia DNA topoisomerase I enzyme, which functions both as a restriction enzyme and as a ligase. iphone defender caseSpletpCR®Blunt II-TOPO®ベクターは、耐熱性プルーフリーディングポリメラーゼ（Platinum® Pfx DNAポリメラーゼなど）で生成された平滑末端PCR産物のクローニングが行えるよ … iphone default warrantySpletZero Blunt TOPO PCR Cloning Kit options The Zero Blunt TOPO PCR Cloning Kit for direct insertion of blunt-ended PCR products into a plasmid vector can be purchased with a variety of competent cells that deliver different advantages depending upon your needs General cloning: TOP10 cells (Cat. No. K2800-20, K2800-40) ... iphone deleted all my picturesSpletZero Blunt TOPO PCR Cloning provides a highly efficient, 5 minute, one-step cloning strategy ("TOPO Cloning") for the direct insertion of blunt-end PCR products into a … iphone deleted message historySpletThe plasmid vector (pCR®-Blunt II-TOPO®) is supplied linearized with Vaccinia virus DNA topoisomerase I covalently bound to the 3´ end of each DNA strand (referred to as "TOPO®-activated" vector). The TOPO®Cloning Reaction can be transformed into chemically competent cells or electroporated directly into electrocompetent cells. iphone default notification toneSpletpCR-Blunt II-TOPO Parental vector for TOPO® cloning of blunt PCR products. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free Download … iphone deleted call history recovery