site stats

Rk2 replicon

WebDec 1, 1992 · The RK2 replicon is not targeted by the pFREE crArray, thus omitting the CRISPR-Cas9-based self-curing feature of the pFREE system but carries a trfA mutant that … WebApr 11, 2024 · In order to slowly approach (and not to exceed) the correct ATPase level, its expression was driven from a low-copy broad-host range (RK2 replicon) vector (Blatny et al., 1997a, 1997b) in the E. coli K-12 MG1655 wild-type strain.

[PDF] The phenotypes of temperature-sensitive mini-RK2 replicons …

WebJul 1, 2016 · The plasmid pGB-hyg-Ptet-gbaA which consists of tetracycline inducible promoter (Ptet), broad-host-range RK2 replicon (oriV and trfA gene), ColE1 origin, … WebThe plasmid vectors described in this report are derived from the broad-host-range RK2 replicon and can be maintained in many gram-negative bacterial species. The complete … barbara engel hannah herzsprung https://airtech-ae.com

CH391L/S12/Origins of Replication - OpenWetWare

WebReplication and incompatibility properties in Escherichia coli of DNA segments from the replication origin region of plasmid RK2 have been investigated. A 393 bp HpaII fragment, … WebJul 20, 2015 · Here, using the plasmid RK2 replicon, we analyze the protein interactions required for Escherichia coli polymerase III (Pol III) holoenzyme association at the … WebThe binary vector pPCV001 (9.2 kbp) has a conditional mini-RK2 replicon (oriV and oriT) and can be maintained in Agrobacterium and E. coli only when co-existed in trans with the trf …puttytel.exe

Improved Broad-Host-Range RK2 Vectors Useful for High

Category:Replication Origin of the Broad Host Range Plasmid RK2

Tags:Rk2 replicon

Rk2 replicon

The host range of RK2 minimal replicon copy-up mutants is

WebJul 21, 2024 · Royalty Kingdom 2. @PlayRK2. Royalty Kingdom 2 on Roblox! Be a queen, a fairy or a fashion icon, but the most important thing is to stand with your own shine! Art … Web中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803

Rk2 replicon

Did you know?

Web暨南大学,数字图书馆. 开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆 </i>

WebKeywords. trfA deletion; oriV RK2 replicon. Introduction Maintenance of a plasmid in a given bacterial host largely depends upon its replication and/or separation proficiency. Among … WebMar 7, 2012 · RK2 plasmids can be used in most gram-negative bacteria. RSF1010 can use used in most gram-negative bacteria, and some gram-positive; ... By definition, chromids …

WebMar 3, 2014 · Bacterial conjugation is a process that is mediated either by a direct cell-to-cell junction or by formation of a bridge between the cells. It is often used to transfer DNA … WebVector is mobilisable via RP4/RK2 mating system, e.g. using E.coli strains S17 or SM10. Note that cloned genes are expressed from the lac promoter, but that this is not …

WebPublished Ahead of Print 25 May 2007. €€ J. Bacteriol.€2007, 189(16):5875. DOI: 10.1128/JB.00385-07. Hernández-Lucas de los Santos, M. F. Dunn, D. W. Emerich ...

WebNov 1, 2010 · The RK2 plasmid is a 60 kbp broad-host-range replicon of the IncPα incompatibility group (Chikami et al., 1985).It was originally identified as an antibiotic … barbara erkinsWebMar 18, 2013 · It is well known that gene dosage and expression levels often correlate, at least to some extent. In order to investigate any potential gene-specific effects related to … putty工具使用WebOur studies have defined a plasmid segment that contains a region which must be linked in cis to the ori RK2 replicon to allow mini RK2 plasmid maintenance in P. aeruginosa PAO … barbara engh obituaryWebNov 1, 2013 · Name of replicon Name of vector constructed Type of vector Antibiotic resistance Size (kb) Reference RK2 pRK290 Cloning vector Tc 20 Ditta et al. (1985) RK2 … putuma new albumWebThe RK2 replicon plasmids produced a level of ir that was equivalent to the wild-type level (pMM4), or 2.25-fold (pPR1A6R1), 4.5-fold (pPR1A6R3), or 9.0-fold (pPR1A6) above … putuoheWebJul 1, 2009 · RK2 replicons potentially eliminate these types of limitations, and large-sized poorly characterized cosmid-RK2-derivatives have been used for metagenomic library … puttyman 下载WebThe 393-base pair minimal origin,oriV, of plasmid RK2 contains three iterated motifs essential for initiation of replication: consensus sequences for binding the bacterial DnaA … barbara erni basel